Journal: Annals of Gastroenterology : Quarterly Publication of the Hellenic Society of Gastroenterology

Article Title: Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis

doi:

Figure Lengend Snippet: Elevated soluble CD14 (sCD14) concentration in the serum of cirrhotic patients. Concentration of sCD14 was measured in healthy controls (n=31), patients with chronic viral hepatitis (n=26) and patients with cirrhosis (n=50) as described in patients and methods. Data is presented graphically as Whisker box-plots

Article Snippet: For sCD14, human CD14 ELISA Development kit (R&D Systems, Abingdon, UK) was used following manufacturer’s instructions.

Techniques: Concentration Assay, Whisker Assay

Journal: Annals of Gastroenterology : Quarterly Publication of the Hellenic Society of Gastroenterology

Article Title: Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis

doi:

Figure Lengend Snippet: High correlation between the levels of human beta defensin-1 (hBD-1) and soluble CD14 (sCD14) in the hepatic veins of cirrhotic patients . Concentrations of hBD-1 and sCD14 were measured as described in patients and methods. Each dot corresponds to individual patients with cirrhosis (n=45). Analysis was performed in samples collected from peripheral veins (n=25, ) and from hepatic veins (n=20, )

Article Snippet: For sCD14, human CD14 ELISA Development kit (R&D Systems, Abingdon, UK) was used following manufacturer’s instructions.

Techniques:

Journal: Annals of Gastroenterology : Quarterly Publication of the Hellenic Society of Gastroenterology

Article Title: Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis

doi:

Figure Lengend Snippet: Soluble CD14 (sCD14) and lipopolysaccharide binding protein (LBP) strongly correlate in serum of patients with cirrhosis. Concentrations of sCD14 were measured as described in Patients and Methods. Concentrations of LBP were measured by a commercially available ELISA, according to manufacturer’s instructions. Each dot corresponds to individual patients with cirrhosis (n=36). Analysis was performed in samples collected from peripheral veins

Article Snippet: For sCD14, human CD14 ELISA Development kit (R&D Systems, Abingdon, UK) was used following manufacturer’s instructions.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Advanced Science

Article Title: Human Platelet Lysates‐Based Hydrogels: A Novel Personalized 3D Platform for Spheroid Invasion Assessment

doi: 10.1002/advs.201902398

Figure Lengend Snippet: Protein and growth factor release from PLMA hydrogels. A) Total protein quantification and B,C) ELISA quantification of TGF‐β1 and VEGF‐A release from PLMA hydrogels at 10, 15, and 20% (w/v). Data are presented as mean ± SD ( n ≥ 3).

Article Snippet: ELISA assays were performed to quantify the release of vascular endothelial growth factor (VEGF Human ELISA Kit, Invitrogen, ThermoFisher Scientific, USA), transforming growth factor β1 (TGF‐β1 Human ELISA Kit, Invitrogen, ThermoFisher Scientific, USA), and epidermal growth factor (Human EGF Quantikine ELISA Kit, R&D systems, Minneapolis, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of food and drug analysis

Article Title: Suppression of ERK1/2 and hypoxia pathways by four Phyllanthus species inhibits metastasis of human breast cancer cells.

doi: 10.1016/j.jfda.2016.03.010

Figure Lengend Snippet: Figure 2 e Protein-expression levels in (A) untreated cells and (B) representative images of cells treated with aqueous Phyllanthus watsonii. (C) Western blot showing VEGF expression in untreated cells and cells treated with extracts from the four Phyllanthus species. (d) Percentage of individual protein expression. (E, F) MMP expression in cells after treatment with aqueous and methanolic extracts from four Phyllanthus species, respectively. ELISA-based detection of (G) iNOS and (H) VEGF expression in untreated cells treated with extracts from the four Phyllanthus species. Error bars indicate the standard error of the mean of three independent experiments. p < 0.05 for each Phyllanthus treatment as compared with the untreated group. APN ¼ aqueous P. niruri; APU ¼ aqueous P. urinaria; APW ¼ aqueous P. watsonii; APA ¼ aqueous P. amarus; C ¼ untreated control; Cis ¼ cisplatin; Dox ¼ doxorubicin; ELISA ¼ enzyme-linked immunosorbent assay; H ¼ 500 mg/mL; I ¼ IC50 dosage; iNOS ¼ inducible nitric oxide synthase; L ¼ 50 mg/mL; M ¼ DNA marker; MMP ¼ matrix metalloproteinase; MPA ¼ methanolic P. amarus; MPN ¼ methanolic P. niruri; MPU ¼ methanolic P. urinaria; MPW ¼ methanolic P. watsonii; VEGF ¼ vascular endothelial growth factor.

Article Snippet: Total iNOS in the four Phyllanthus species-treated cells was measured using a human total-iNOS immunoassay enzymelinked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer instructions.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Control, Marker

Journal: Pediatric Reports

Article Title: Resistin in Urine and Breast Milk: Relation to Type of Feeding and Anthropometry at 1-Month

doi: 10.3390/pediatric14010013

Figure Lengend Snippet: Correlation between resistin concentration in the breast milk and ( a ) body weight; ( b ) age; ( c ) body mass index (BMI); and ( d ) leptin concentration in mothers’ breast milk at 1 month post-partum.

Article Snippet: We analyzed urinary and breast milk resistin levels (Human Resistin ELISA kit; Arigo biolaboratories, Hsinchu City, Taiwan) and breastmilk leptin (Human Leptin Quantikine ELISA Kit; R&D Systems, Minneapolis, MI, USA) in infants using an enzyme-linked immunosorbent assay kit.

Techniques: Concentration Assay

Journal: Neuron

Article Title: Edinger-Westphal peptidergic neurons enable maternal preparatory nesting

doi: 10.1016/j.neuron.2022.01.012

Figure Lengend Snippet:

Article Snippet: For circulating Progesterone titration, a drop of blood was sampled from the mouse cheek, and processed through a Mouse Progesterone ELISA kit according to the manufacturer’s instructions (Novus Biological NBP2-60125-1).

Techniques: Virus, Recombinant, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Software